Apoptosis of gastric mucosal cells has also been studied under varied conditions in rats, including ethanol, nonsteroidal antiinflammatory drugs, retinoic acids, h pylori infection2630. The gastric mucosal barrier and ulceration sciencedirect. Gastric ulceration induced by nonsteroidal antiinflammatory drugs is a neutrophildependent process. Home archives vol 2 no 1 2014 articles gastric mucosa reepithelisation, oxidative stress and apoptosis during healing of acetic acidinduced ulceration in thyroxine treatment and thyroidectomy on rats. Neutrophils have been implicated in the pathogenesis of gastric mucosal damage induced by h. Gastric lesion score in ethanolinduced gastric mucosal. A novel antioxidant and antiapoptotic role of omeprazole to block. Gastric irritantinduced apoptosis in guinea pig gastric. Increased apoptosis in gastric mucosa adjacent to intestinal.
Pathogens 20, 2 573 the aims of this study were to investigate the effects of a selective egfr tyrosine kinase inhibitor, ekb569 30 on h. Normal gastric mucosa contained low numbers of apoptotic cells at the. Apoptosis is a major mechanism of deoxycholateinduced. A partner for gastric ulceration article in zhonghua yi xue za zhi chinese medical journal. The animals receiving 96% ethanol developed a consistent pattern of macroscopic damage, as evidenced by the presence of. Gastric ulcers were induced by absolute ethanol 5 mlkg, i. The relation between the level of apoptosis in mucosal cells examined by flow cytometry and the. The animals receiving 96% ethanol developed a consistent pattern of macroscopic damage, as evidenced by the presence of hemorrhagic ulceration figure 3a1. The availability of new antibodies for the detection of apoptotic cells in tissue sections has facilitated the analysis of the role. In the absence of antiulcer agents, ethanol caused extensive mucosal lesions accompanied. Chronic gastritis induced by helicobacter pylori is a strong risk factor for thedevelopment of distal gastric adenocarcinoma. In humans, it is about 1 mm thick, and its surface is smooth, soft, and velvety.
Muscimol microinjection into cerebellar fastigial nucleus. The pathophysiology of nonsteroidal antiinflammatory drug nsaid induced mucosal injuries in stomach and small intestine. Melatonin is a hormone synthesized from serotonin in the mammalian pineal gland, the retina, lymphocytes, gi tract, and bone marrow. Pretreatment with ga could inhibit the apoptosis of gastric mucosal cells. Epithelial cell proliferation was scored by use of immunoperoxidase staining with mib1 antiki67 antibody by a single pathologist blinded to h. Gastric irritantinduced apoptosis in guineapig gastric. In the gastric epithelium, apoptosis plays an essential role in maintaining tissue integrity. Pdf helicobacter pylori and peptic ulcer role of reactive oxygen. Gastric irritantinduced apoptosis in guineapig gastric mucosal cells in primary culture shinji tsutsumi a, wataru tomisato, tatsunori takano, kazuhito rokutan b, tomofusa tsuchiya a, tohru mizushima. Folic acid supplementation ameliorates inflammation and. Recent studies also indicate that programmed cell death or apoptosis plays a significant role in gastric ulcer ation. The mechanisms involved in the pathogenesis of a new model for experimental gastric ulcer after epinephrine administration to the rat were evaluated. Acute toxicity and gastroprotective effect of 2pentadecanone in ethanolinduced gastric mucosal ulceration in rats. Molecular pathogenesis of gastric ulcers diseases and strategies for prevention.
Biochanin a gastroprotective effects in ethanolinduced gastric mucosal ulceration in rats. Gastric mucosal ulceration after epinephrine springerlink. Gastric mucosal inflammation and epithelial cell turnover. Gastric epithelial cell proliferation and apoptosis in helicobacter. Biochanin a gastroprotective effects in ethanolinduced gastric. In fact, duodenogastric reflux is thought to play a major role in the pathogenesis of human gastric ulceration, and bile seems to be a key participant in this process 8, 30. Furthermore, a significant increase in apoptosis of the gastric mucosal cells was identified. The gastric mucosal barrier is the property of the stomach that allows it to safely contain the gastric acid required for digestion if the barrier is broken, as by acetylsalicylic acid asa, aspirin in acid solution, acid diffuses back into the mucosa where it can cause damage to the stomach itself the barrier consists of three protective components. The number of vacuoles was increased in the gastric mucosal cells. Folic acid supplementation ameliorates inflammation and apoptosis in ethanolinduced gastric ulceration in rats. Trend analysis revealed that ag treatments inhibited ethanolinduced gastric mucosal damage. Proulcerative exogenous factors which enhance oxidative stress through over.
In both cases, a decrease in endothelial cell proliferation and an increase in apoptosis were observed. Hence, gastric mucosal integrity is maintained by interplay of aggressive and defensive factors controlling papo tosis and cell proliferation. In a second experiment, animals were pretreated orally with each treatment for 1 hr before a single oral administration of ethanol 70%, 10 mlkg. Interplay between helicobacter pylori and immune cells in. Exercise stress, intestinal permeability and gastric. The influence of the vasoactive catecholamines, epinephrine and norepinephrine, has been investigated in regard to the comparative ulcerogenicity on the gastric glandular mucosa of the rat. Prostaglandin e2 protects gastric mucosal cells from apoptosis via. Mechanism of apoptosis involved in gastric mucosal lesions. Folic acid ameliorates the development of gastric ulcer in rats via its anti inflammatory and antiapoptotic activities.
The gastric mucosa is the mucous membrane layer of the stomach, which contains the glands and the gastric pits. Effect of aging on gastric mucosal defense mechanisms. The biological processes involved in the development of gastric mucosal atrophy and intestinal metaplasia are still incompletely understood. Gastric mucosal injury and oxidative stress sciencedirect. The oral intubation of ethanol induced gastric ulceration in the glandular region of the gastric cavity, as indicated by an average gui of 39.
The results of previous studies have shown that apoptosis in mucosal cells of the small intestine was increased in old rats. Normally, the rate of cell loss by apoptosis is matched by the rate of cell proliferation. The clinical consequences range from asymptomatic gastritis to peptic ulceration and gastric malignancy. Potential role of probiotics in the management of gastric. Helicobacter pylori, inflammation, mucosal damage, and apoptosis. Pdf folic acid supplementation ameliorates inflammation and. Selected references these references are in pubmed. Role of apoptosis induced by helicobacter pylori infection in the development of. Furthermore, the activation of nrf2 produces the ho1 upregulation, protecting the gastric mucosa by inhibiting apoptosis. Cyclooxygenase 2implications on maintenance of gastric mucosal integrity and ulcer healing. The intrinsic apoptotic signaling pathway in gastric. Major histocompatibility complex class ii inhibits fas. Gastric mucosal ulceration following vasoactive agents. The expression of phosphatase and tensin homolog was significantly higher in gastric mucosa from patients with hapcinduced gml compared with that in the healthy controls.
Mucosal apoptosis induced by ischemiareperfusion and. Backgroundinfection with a virulent helicobacter pylori strain is associated with gastric mucosal damage and the increased risk of gastric cancer aimsto examine the characteristics of host gastric mucosal responses in patients with gastric cancer, histological grade of gastritis, gastric epithelial apoptosis, and proliferation were studied. Epidermal growth factor and prostaglandin e 2 accelerate mucosal recovery from stressinduced gastric lesions via inhibition of apoptosis. Mucosal apoptosis induced by ischemiareperfusion and fasting in the stomach compared to the small intestine in rats author. Gastric ulcers diseases pathogenesis, complications and strategies for prevention. Cyclooxygenase 2implications on maintenance of gastric.
Gastric mucosal defect could result from several causative factors including the use of nonsteroidal antiinflammatory drugs, helicobacter pylori infection, gastrointestinal and spinal cord diseases, and neoplasia. It consists of simple columnar epithelium, lamina propria, and the muscularis mucosae. Folic acid ameliorates the development of gastric ulcer in rats via its antiinflammatory and antiapoptotic activities. To investigate the effects of microinjection of the gabaa receptor agonist muscimol into cerebellar fastigial nucleus fn on stressinduced gastric. The use of the term gastric mucosal barrier has often been misconstrued to suggest that this tissue is impenetrable. Li li department of pathophysiology, xuzhou medical college, xuzhou 221002, jiangsu province, china. The unique extensive, hemorrhagic necrosis, and ulcerations which appear in the fundusbut not the antrumfollowing single injections of these drugs are readily quantified because of the continuous. Apoptosis, programmed death of cells is able to activate by consump. This study was performed to achieve a novel simple, inexpensive, and effective surgical technique for the repair of gastric mucosal defect. The full text of this article is available as a pdf 160k. Indomethacininduced apoptosis, ulcer, gastric, rgm1 cell line introduction 0f 1 gastric mucosal damage occurs as a result of imbalance between aggressive factors such as hydrochloric acid, helicobacter pylori, pepsin and gastrin as well. Apoptosis in helicobacterpyloriassociated gastric and duodenal. Biochanin a gastroprotective effects in ethanolinduced.
Ulcer prevention effect of 3,4,5tihydroxyn02methyl1hindol3ylmethylidenebenzohydrazide in hclethanolinduced gastric mucosal damage in rats. Molecular pathogenesis of gastric ulcers diseases and. In fact, ulcer disease is a cell loss disorder occasioned by the stimulation of apoptosis or inhibition of cell proliferation 6. Although the gastroprotective potential of folic acid has been reported, little is known about the role of inflammation and apoptosis in the said activity. Gastropathy, such as gastric ulcer and gastritis, is caused by damage to the gastric mucosa due to its exposure to various gastric irritants such as. Confluent rat gastric mucosal rgm1 cells were challenged with medium containing 500um indomethacin in the presence or absence of 0. Cytoprotective effect of american ginseng in a rat ethanol. Effects of hypothalamic paraventricular nuclei on apoptosis and proliferation of gastric mucosal cells induced by ischemiareperfusion in rats. Helicobacter pylori and peptic ulcer role of reactive. Gastric ulcers, characterized by mucosal damage, are induced by.
Similarly, astaxanthin has been shown to provide protection against naproxeninduced and stressinduced gastric ulceration by reducing the. Reports testing the hypothesis that apoptosis leads to atrophy have yielded conflicting results. The gastric mucosal barrier is that property which defends against acid and which impedes diffusion of acid from the lumen into the mucosa. Role of apoptosis induced by helicobacter pylori infection. Ulcer prevention effect of 3,4,5tihydroxyn02methyl. Gastric mucosa reepithelisation, oxidative stress and.
Pdf biochanin a gastroprotective effects in ethanol. Nsaids are two major causes of gastric ulceration, but relation between h. Novel approach to gastric mucosal defect repair using. Homeostasis of the gastrointestinal mucosa is maintained. The aim of this study was to investigate the capacity of h. Effects of egfr inhibitor on helicobacter pylori induced. This study, therefore, assessed lipid peroxidation lpo, neutrophillymphocyte ratio nlr, creactive protein crp as markers of inflammation and, p53 and bcl2 as markers of apoptosis in ethanolinduced gastric ulcer pretreated with. Experimental studies suggest that probiotics could contribute to the prevention and therapeutic modalities of gastric ulcer by enhancing. Gastric mucosa definition of gastric mucosa by medical. Analysis of apoptotic and antiapoptotic signalling pathways. Read characterization of naproxen induced gastric antral ulceration and adaptation in hamsters, gastroenterology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Gastric lesion score in ethanol induced gastric mucosal.
It plays an important role as antioxidant, property that is very important in many. Momordica charantia polysaccharides ameliorate oxidative. Gastroprotective effect of gallic acid against ethanolinduced gastric. Some reports of gastric mucosal cellular apoptosis induced by gastric ir are contradictory. The pathophysiology of nonsteroidal antiinflammatory. Gastric mucosal lesions caused by stress, indomethacin, ethanol, and h. Ag supplementation dosedependently decreased the proinflammatory levels of. Since apoptosis has been identified as a potent factor in ulcerogenesis, the study was designed to examine the antiapoptotic effect of kv in intact and cultured gastric cells rgm1 of rat. Gastric ulcers diseases pathogenesis, complications and.
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